Characterization of recombinant traceable analogs of the parathyroid hormone
نویسندگان
چکیده
منابع مشابه
Purification and characterization of recombinant human parathyroid hormone-related protein.
Full-length human parathyroid hormone-related protein (PTHrP-(1-141] as well as a carboxyl-terminal shortened form (PTHrP-(1-108] have been expressed from recombinant DNA-derived clones. These proteins were expressed in Escherichia coli as fusion proteins so that cyanogen bromide cleavage yields the desired product. Both proteins were purified and then characterized by sodium dodecyl sulfate ge...
متن کاملRecombinant human parathyroid hormone 1-84 (Natpara)
Description/Mechanism of Action Recombinant human parathyroid hormone (rhPTH 1-84) is identical to endogenous parathyroid hormone (PTH) and binds PTH-1 receptors in the bone, kidney, and has an indirect effect on calcium reabsorption in the intestine. It increases serum calcium by increasing renal tubular calcium reabsorption, intestinal calcium absorption, and bone turnover. Indication under R...
متن کاملIsolation and Characterization of Bovine Parathyroid Hormone*
It has been known for some time that many active principles found in tissues are difficult to extract and purify, not only because of problems of stability, but also because they often apljear to bc bound, in some more or less specific way, with other tissue components. Strong dissociating solvents may be required to achieve disentanglement from other components, but such solvents must not caus...
متن کاملCharacterization of Parathyroid Hormone Fragments Produced
Cleavage of parathyroid hormone by cathepsin D was studied. Four primary products were detected and separated by high performance liquid chromatography. Two of the fragments are fluorescent and therefore contain residue 23 (tryptophan). These fragments are NHderminal in origin. The other two cross-react with antisera directed against COOH-terminal portions of the hormone; they are the complemen...
متن کاملIsolation and characterization of bovine parathyroid hormone.
It has been known for some time that many active principles found in tissues are difficult to extract and purify, not only because of problems of stability, but also because they often apljear to bc bound, in some more or less specific way, with other tissue components. Strong dissociating solvents may be required to achieve disentanglement from other components, but such solvents must not caus...
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ژورنال
عنوان ژورنال: The FASEB Journal
سال: 2013
ISSN: 0892-6638,1530-6860
DOI: 10.1096/fasebj.27.1_supplement.1170.1